Composite
Part:BBa_K1022128
Designed by: Maithili Krishnan Group: iGEM13_TU-Delft (2013-09-20)
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
This biobrick is the one of the three final Intergrated constructs of the project Peptidor by Team TU Delft iGEM 2013. This has a Promoter pBAD with the AIP receiver to sense the AIPs followed by a His6 SUMO tagged peptide along with a TetR which suppresses the timer module containing promoter pTET, cI is produced which suppresses pcI. Then Ulp protease is produced following by a Kill switch lysis device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 2317
Illegal NheI site found at 4609
Illegal NheI site found at 6704
Illegal NheI site found at 6727
Illegal NotI site found at 5305 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 521
Illegal BglII site found at 2441
Illegal BamHI site found at 65
Illegal BamHI site found at 1405
Illegal BamHI site found at 5274
Illegal XhoI site found at 5283
Illegal XhoI site found at 5314 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2626
Illegal AgeI site found at 6309
Illegal AgeI site found at 6379 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 6960
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Categories
Parameters
None |