Reporter
Part:BBa_K1094400
Designed by: Emil Christian Fischer Group: iGEM13_UNIK_Copenhagen (2013-09-14)
Revision as of 21:33, 2 October 2013 by Emilfischer (Talk | contribs)
Enhanced green fluorescent protein (eGFP)
A modified version of the green fluorescent protein (GFP) derived from Aequorea victoria with enhanced fluorescence properties. The sequence can be used as either reporter or fluorescent tag.
The part was cloned into the expression vector pJAM1786 via pDONR207 in E. coli, by using the Gateway system by Invitrogen. Colony PCR and gel electrophoresis was performed to ensure the insert was in the vector (shown in the seven leftmost lanes of fig. 1).
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