Not Released
Sample In stock
Experience: Works
Not Used
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Project
c-di-AMP

Part:BBa_K1045002

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-06-24)
Revision as of 11:59, 25 September 2013 by Nina Heckmann (Talk | contribs)

CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch

This part is a reporter system. It was constructed from a riboswitch from Bacillus subtilis with a native promoter and native rbs (part BBa_K1045004) and a CFP reporter. It works as a sensor of c-di-AMP, which enhances the terminating efficiency in the translation of the RNA by ribosomes. Therefore in the presence of c-di-AMP the system should be hindered and not be glowing. In combination with the diadenylate cyclase it might show further interaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rna/riboswitch
Parameters
None