1 Registry Star
Generator
PchA/PchB
Part:BBa_J45319:Experience
Designed by: André Green II Group: iGEM06_MIT (2006-10-28)
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how you used this part and how it worked out.
Applications of BBa_J45319
Enzymes generator for catalyzing production of salicylic acid from the cellular metabolite chorismate.
User Reviews
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BBa_J45319, when used in the wintergreen odor biosynthetic system BBa_J45700, appears to produce some salicylate from the cellular metabolite chorismate. |
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Characterization
Gas chromatography analysis of wintergreen odor biosynthetic system
The wintergreen odor biosynthetic system (BBa_J45700) includes the PchA & PchB enzyme generator (BBa_J45319) under the control of a constitutive promoter (BBa_R0011).
Verification of methyl salicylate production from cellular metabolites by wintergreen biosynthetic system
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.