Coding

Part:BBa_K1159001

Designed by: TU Munich 2013   Group: iGEM13_TU-Munich   (2013-05-17)
Revision as of 16:33, 15 September 2013 by Earthnut (Talk | contribs) (Functional Parameters)

NanoLuc Luciferase in RFC[25]

NanoLuc Luciferase is engineered ATP-independent luciferase from a deep-sea shrimp which luminescense 2 magnitues higher than these from Renilla reniformis or from Photinus pyralis (firefly). Also the molecular weight of NanoLuc luciferase is twice smaller compared to other luciferase (only 19 kDa).


Usage and Biology

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

[edit]
Categories
//cds/reporter
//chassis/eukaryote/ppatens
//function/reporter/light
Parameters
None