Help:Standardization

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What you've got: Any non-Biobrick-standardized sequence of DNA that does something interesting
What you want:A Biobrick standardized sequence of interesting DNA

So here's how you do it...

  1. First off, check your DNA sequence for restriction sites. This is easy using available web programs such as
    • [http://tools.neb.com/NEBcutter2/index.php NEB cutter]
    • [http://wishart.biology.ualberta.ca/PlasMapper/ PlasMapper] - developed by the University of Alberta
  2. Next, locate and remove all of the BioBrick standard restriction sites (XbaI, SpeI, EcoRI, PstI) that are on your sequence. To delete these sites, you can use