Help:Plasmid backbones/Nomenclature
Plasmids in the Registry of Standard Biological parts adhere to a particular naming convention. This page describes the details of this naming scheme.
If you are interested in designing and/or constructing a new BioBricks compatible plasmid and are submitting it to the Registry, please follow this naming convention. Questions or comments can be directed to Reshma Shetty.
Contents
pSBxyz-a
pSB = plasmid Synthetic Biology
x = origin of replication
y = selection marker
z = version number
-a = insert
Origin of replication
- 1= pUC19-derived pMB1 (in pSB series)
- 2 = pSCANS-derived
- 3 = p15A pMR101-derived
- 4 = rep101, repA pSC101-derived
- 5 = F plasmid
- 6 = pBR322
Selection marker
- X = none
- A = ampicillin
- C = chloramphenicol
- E = erythromycin
- G = gentamycin
- K = kanamycin
- N = neomycin
- Na = nalidixic acid
- R = rifampicin
- S = spectinomycin
- St = streptomycin
- T = tetracycline
- Tm = trimethoprim
- Z = zeocin
- AK = kanamycin and ampicillin
- KT = kanamycin and tetracycline
- AKT = ampicillin and kanamycin and tetracycline
- AC = ampicillin and chloramphenicol
… etc
Version number
(used to indicate miscellaneous interesting changes to backbone)
- 0 = nothing
- 1 = complete Biobricks insertion site
- 2 = 5’ terminator and complete Biobricks insertion site
- 3 = 5’ terminator and complete Biobricks insertion site and 3’ terminator
- 4 = misc restriction sites removed for the T7 project
- 5 = Held for next generation of cloning plasmid. See Synthetic Biology:Vectors/Parts.
- 10 = Held for a version of Jason's measurement plasmid
- 11 = Held for a version of Jason's measurement plasmid
- 12 = Held for a version of Jason's measurement plasmid
Insert
- generally a BioBricks part number where multiple parts are separated by periods
- no dash = no insert
- -P1010 = ccd operon insert
- -1 = ccdB <-- this has now become legacy nomenclature.
Contact
Questions or comments can be directed to Reshma Shetty.