Software error:
Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 248, near "range;" Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 249, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 249, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 259, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 280, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 286. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 312, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 10. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 10. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9.
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Status: 500 Content-type: text/html
Software error:
Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 248, near "range;" Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 249, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 249, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 259, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 280, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 286. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 312, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Status: 500
Content-type: text/html
Software error:
Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 248, near "range;" Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 249, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 249, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 259, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 280, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 284, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 286. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 312, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Design Notes
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature.
See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki].
Source
De novo design.