Regulatory
pBAD
Part:BBa_K845001
Designed by: Grégory Hansen Group: iGEM12_Grenoble (2012-09-26)
Revision as of 16:52, 29 September 2012 by Greghansen (Talk | contribs)
New paraBAD part
iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).
In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.
Usage and Biology
paraBAD is a promoter which can be activated by 2 molecules : CRP-cAMP complex and the AraC protein. It has two states; in absence of L-arabinose the paraBAD promoter is repressed by AraC, whereas the paraC promoter is activated (unless an excess of AraC is present).
In presence of L-arabinose and the CRP-cAMP complex, the promoter is activated thus enabling the transcription of the downstream elements.
This AND gate provides a filter to biological noise.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 344
Illegal XhoI site found at 494 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 179
Illegal AgeI site found at 484 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 161
[edit]
Categories
Parameters
//promoter
| negative_regulators | AraC protein |
| positive_regulators | cAMP-CRP complex and Arabinose |