Coding
cyaA

Part:BBa_K845008

Designed by: Grégory Hansen   Group: iGEM12_Grenoble   (2012-09-26)
Revision as of 16:31, 29 September 2012 by Greghansen (Talk | contribs)

cyaA with its natural RBS

cyaA gene (from BW25113 E. Coli) encodes the Adenyl cyclase protein which catalyses the conversion of ATP (Adenosine Tri-Phosphate) into cAMP (cyclic Adenosine Mono-Phosphate).

Our saferty note: https://static.igem.org/mediawiki/2012/4/48/CyaA.pdf


Usage and Biology

In Escherichia coli, cAMP is involved in carbon catabolite repression [1] and binds to the cAMP receptor protein (CRP). The corresponding complex (CRP-cAMP) is a transcriptional factor controlling the expression of more than 220 operons [2]. It has been known for a long time that E. coli actively exports cAMP into the growth medium [3][4].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 288
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 922
    Illegal SapI.rc site found at 412
    Illegal SapI.rc site found at 652


[edit]
Categories
//cds/enzyme
Parameters
None