Coding

Part:BBa_K942004:Experience

Designed by: Luis Mario Leal Garza   Group: iGEM12_Tec-Monterrey   (2012-09-26)
Revision as of 03:23, 27 September 2012 by Wishomario (Talk | contribs) (→‎Applications of BBa_K942004)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K942004

This protein was expressed in Pichia pastoris. Thanks to its B.P.S. it was also planned to be expressed in Escherichia coli. We succesfully transformed the gene into our cloning strains, but curiously, our expression strains (DE3 strains) were not even able to be transformed, except from Rosettagami DE3 pLys. We believe that the fact that Rosettagami had pLys to prevent leak expression and the other expression strains did not, leads us to the idea that there might be posibility of Der f 2 having antibacterial effects, as it is describe on the main page of this same part.

We were able to se the expression of this protein on a SDS-PAGE after purifying it with a His-tag affinity column. Currently, we are trying different conditions to achieve the expression in BL21 Star.

User Reviews

UNIQ9ea9c91eb22597a6-partinfo-00000000-QINU UNIQ9ea9c91eb22597a6-partinfo-00000001-QINU