Device

Part:BBa_K771002

Designed by: WU Yuqi;YIN Ruonan;GUO Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)
Revision as of 06:05, 28 September 2012 by AleAlejandro (Talk | contribs)

Membrabe Anchor 2:ssDsbA-Lgt-SH3 Domain-MS2

Membrane Anchor 2:RNA aptamer binding protein MS2 fused with membrane protein Lgt. And it is part of Membrane Rudder system.

Membrane Rudder System

12SJTU-MA1-MA6.jpg

Demonstration of the whole Membrane Rudder device. When RNA signal molecule is present, Membrane Anchor 1 and 2 dimerize, so enzyme 1, 2, 3 ,4 and 5 aggregate to activate one of the pathways.

RNA Rudder

Membrane Anchor 1 and 2 act as a controlling point to determine whether certain pathway (in this case involving enzyme 1, 2, 3, 4 and 5) is activated or not.

12SJTU RNASIGNAL.jpg

A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding enzymes.

Related Biobrick

<br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 5([BBa_K771005]) <br\>Membrabe Anchor 6([BBa_K771006])



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 504
    Illegal BsaI site found at 1609
    Illegal SapI.rc site found at 1150


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Categories
Parameters
n/aMembrabe Anchor 2:ssDsbA-Lgt-SH3 Domain-MS2