Device

Part:BBa_K812014

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-18)
Revision as of 01:23, 27 September 2012 by Chr.karine (Talk | contribs)

Auxin production device for expression in tadpole We adapted the biobrick BBa_515100 made by the iGEM team imperial college to the eucaryotes.

This device is ment for the co-expression of IaaH and IaaM gene in the same cell in tadpole. The creation of polysistronic mRNA in tadpole doesn't work very well. This is why the two proteins are fused with a self cleavable peptide domain Pep2A which allow the protein to be transcribed as a single peptide that is after cleaved in two different proteins that fold later on.

This part is to create auxin in tadpole as the emmitor device of our sythetic hormonal system. Have a look at our wiki to undersand how this system is supposed to work: http://2012.igem.org/Team:Evry/FrenchFrog.

This part can be used in an oviparian plasmid such as pSC2+ (see K812000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1816
    Illegal BamHI site found at 2761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1021
    Illegal NgoMIV site found at 1523
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None