Generator

Part:BBa_K738002

Designed by: Huachun Liu   Group: iGEM12_ZJU-China   (2012-08-09)
Revision as of 18:03, 25 September 2012 by Smile (Talk | contribs)

Theophyline riboswitch regulated RNA Scaffold (clover vision 2)

Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins. A designed theophylline aptamer was added on the original scaffold D0(Media:BBa_K738002) in order to produce an interaction with MS2 aptamer in the absence of theophylline, thus disturbing the bind of MS2 aptamer and corresponding protein.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rna/aptamer
Parameters
n/aTheophyline riboswitch regulated RNA Scaffold