Coding
Cel5A

Part:BBa_K805011:Design

Designed by: Liu Ping   Group: iGEM12_HUST-China   (2012-09-20)
Revision as of 11:09, 26 September 2012 by HUST XueYu (Talk | contribs) (→‎References)

Cel5A, endoglucanases


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 763
    Illegal AgeI site found at 1027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1024


Design Notes

sequence confirmed

Source

Extracted from host genome Bacillus pumilus ARA

References

Qu W, Shao W,Cloning, expression and characterization of glycoside hydrolase family 11 endoxylanase from Bacillus pumilus ARA.Biotechnol Lett. 2011 Jul;33(7):1407-16. Epub 2011 Mar 3.