Plasmid_Backbone

Part:BBa_K801001:Design

Designed by: Georg Schtzinger   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 09:30, 21 September 2012 by Giergl (Talk | contribs) (→‎Source)

pTUM101 yeast shuttle vector with pTEF1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3771
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
    Illegal NotI site found at 42
    Illegal NotI site found at 514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 261
    Illegal BsaI.rc site found at 1928
    Illegal BsaI.rc site found at 2874
    Illegal SapI site found at 845
    Illegal SapI site found at 3026


Design Notes

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible


Source

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

References