Regulatory

Part:BBa_K819017:Design

Designed by: YU Zhou   Group: iGEM12_Peking   (2012-09-16)
Revision as of 16:52, 25 September 2012 by Zhangzidong (Talk | contribs) (→‎Source)

Luminesensor repressible SulA408 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original SulA with the binding site of CTGT(N)8ACAG is mutated to CCGT(N)8ACGG, which is the 408 form.
The LexA 408 protein has corresponding mutations of PA40, NS41 and AS42.


Source

SulA promoter sequence was from [http://regulondb.ccg.unam.mx/gene?organism=ECK12&term=ECK120000973&format=jsp&type=gene regulon]

References