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Part:BBa_K731480

Designed by: Andrea Tassinari, Francesco Guzzonato   Group: iGEM12_UNITN-Trento   (2012-08-22)
Revision as of 14:59, 30 August 2012 by Lmartini (Talk | contribs)

IPTG Inducible sfGFP tagged Cysteine desulfhydrase (CysDes)

A sfGFP tagged CysDes inducible by IPTG.


Usage and Biology

CysDes is a unique 45 KDa hemolysin cysteine dependent, that was shown to have also aminostransferase activity. (1, 2) The enzyme catalyze the degradation of L-cysteine to produce hydrogen sulfide, ammonia and pyruvate. (FIGURE con pathway)

This part produces high levels of CysDes enzyme upon IPTG induction. Protein expression levels have been monitored with the sfGFP tagged composite part BBa_K731480.

Part BBa_K731400 has been fully characterized in psB1C3 and also in the low copy vector psB4K5 using E.coli strain NEB10b.

Please note that this part produces hydrogen sulfide, which is toxic if inhaled in high concentrations. Cells handling should be done under a chemical hood.

Andrea2.jpg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2460
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1559
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2490


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