Part:BBa_K823026

pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has a ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. The multiple cloning site is downstream of a Pspac promoter, that is inducible with IPTG. (Isopropyl-β-D-thiogalactopyranosid).

This BioBrick is part of the LMU 2012 igem project Bacillus BioBrickBOX http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 8268
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 8274
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 8268
  Illegal BglII site found at 5862
  Illegal BamHI site found at 1378
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 8268
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 8268
  Plasmid lacks a suffix.
  Illegal XbaI site found at 8283
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 2925
  Illegal AgeI site found at 5473
  Illegal AgeI site found at 6435
  Illegal AgeI site found at 7110
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2749
  Illegal BsaI.rc site found at 4188
  Illegal BsaI.rc site found at 6704
  Illegal SapI site found at 1666
  Illegal SapI.rc site found at 5686