Coding

Part:BBa_K559000

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM11_Hong_Kong-CUHK   (2011-09-29)
Revision as of 21:32, 5 October 2011 by Jacky Loo (Talk | contribs)

Halorhodopsin

This Halorhodopsin gene allow the its membrane protein to be expressed. It regulates the chloride ion pumped from extracellular to intercellular space, with the rate being controlled by light intensity and quality as well as light exposure time.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 280
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 292
    Illegal AgeI site found at 18
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 228



Usage and Biology

The figure illustrates the molecular mechanism of halorhodopsin gene on chloride ion absoption, in a controlled manner under light illumination.

HR mach.png

1. Essen, L.O. Halorhodopsin: light-driven ion pumping madesimple? Current opinion in structural biology 12, 516–522(2002)

[edit]
Categories
//cds/membrane/channel
//chassis/prokaryote/ecoli
//function/sensor/light
Parameters
biology
function
protein