Translational_Unit

Part:BBa_K578005

Designed by: Kylie Standage-Beier   Group: iGEM11_Arizona_State   (2011-09-28)
Revision as of 22:12, 5 October 2011 by Ksstanda (Talk | contribs) (→‎Confirmation of Function)

J23102+E0840 (Constitutive GFP)

This is a combination of the GFP coding region (E0840), and the medium strength constitutive promoter, J23102.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 708

Confirmation of Function

BBa_K578005 was successfully subcloned into a variety of vectors, including pSB1C3, pSB1A3, pSB1K3, and pRSFDuet-1 (Novagen). The green fluorescent phenotype was observed. The image below show transforments of BL21(DE3) on the right and K12 MG1655 on the left, both with BBa_K578005 in pRSFDuet-1. Both show visible constitutive expression of GFP.

ASU GFP Duet.png

Use in Competent Cells

E. Coli MG1655 with this BioBrick in pRSF Duet was successfully made chemically competent using the OWW TSS procedure, allowing for the transformation of a second plasmid.


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