Reporter
Part:BBa_K598001:Design
Designed by: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG Group: iGEM11_Peking_R (2011-09-27)
Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 71
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 730
Design Notes
The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
Source
Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.