Reporter

Part:BBa_K598007

Designed by: Siyang HAO   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 15:15, 27 September 2011 by Registry (Talk | contribs)

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Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015

It is a theophylline responsive riboswitch containing an original ribosome binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731


[edit]
Categories
Parameters
chassisE. coli DH5α
functionused to test the ability of theophylline responsive riboswitch by GFP fluorescence intensity
ligandstheophylline
n/aTheophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015
rbsmedium
resistanceChloramphenicol