RNA

Part:BBa_K598006:Design

Designed by: Siyang HAO   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 06:19, 3 October 2011 by Hsy (Talk | contribs) (→‎Design Notes)

Theophylline Responsive Riboswitch 1G1 with Engineered RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed to up-regulate downstream gene expression in the presence of theophylline. In order to obtain this part for further utilization,get it by PCR, rather than enzyme digestion.

Source

By PCR.

References