Plasmid
pJEJAM4

Part:BBa_K678052

Designed by: DTU-Danmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-13)
Revision as of 21:21, 20 September 2011 by Jaide (Talk | contribs)

pJEJAM4

pJEJAM4 is a plasmid intended for transient transfection of mammalian cells. pJEJAM4 consists of the strong constitutive cytomegalovirus (CMV) promoter, mCherry, the BGK polyA, the hygR marker cassette, an ori for propagation in E.coli and, ampR resistance cassette (Figure 1).



DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM4, the figure is not drawn to scale.




DTU-Denmark-2 2011 Figure 2: .
DTU-Denmark-2 2011 Figure 3: .


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
    Illegal NgoMIV site found at 3062
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote
//plasmid
Parameters
chassisU-2 OS