Reporter
fLuc

Part:BBa_K518000

Designed by: Masato Ohgishi   Group: iGEM11_UT-Tokyo   (2011-09-15)
Revision as of 07:01, 15 September 2011 by Registry (Talk | contribs)

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RBS + firefly luciferase + d.terminator

Luciferase from firyfly (Photinus pyralis). Firefly luciferase emits light by the oxidation of D-luciferin, its substrate. Luciferase can be used as a measuring tool when combined with other cis-elements (regions regulating the expression of genes on the same DNA molecule; promoters and other protein-binding domains), because of its highly quantitative performance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 827


[edit]
Categories
//classic/reporter
//function/reporter/light
Parameters
emission556nm