Composite

Part:BBa_K611009

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-18)
Revision as of 23:51, 18 September 2011 by Registry (Talk | contribs)

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TetR Characterization Construct

This part is used to characterize mutants of BBa_C0040 (TetR). By adding a mutant of BBa_C0040 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1070
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


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Categories
Parameters
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