Part:BBa_K516220:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K516220

User Reviews

UNIQ0ba2e6c8898df40c-partinfo-00000000-QINU UNIQ0ba2e6c8898df40c-partinfo-00000001-QINU

UNIQ0ba2e6c8898df40c-partinfo-00000002-QINU

•••••

UNIPV-Pavia iGEM 2011

NB: unless differently specified, all tests were performed in E. coli MGZ1 in M9 supplemented medium at 37°C in low copy plasmid pSB4C5.

Characterized with:

BBa_K516220 (wiki name: E24 ) p_Tet-RBS30-AiiA-TT
BBa_K516221 (wiki name: E25 ) p_Tet-RBS31-AiiA-TT
BBa_K516222 (wiki name: E26 ) p_Tet-RBS32-AiiA-TT
BBa_K516224 (wiki name: E27 ) p_Tet-RBS34-AiiA-TT

The AiiA enzyme activity has been characterized under the regulation of p_tet promoter, assaying its enzymatic activity.
The four measurement parts p_Tet-RBSx-AiiA-TT were assayed by BBa_T9002 biosensor.
No HSL degradation was observed for the tested devices in low copy plasmid pSB4C5 after 21 h in M9 medium with pH=7.0.

UNIQ0ba2e6c8898df40c-partinfo-00000005-QINU