Regulatory

Part:BBa_K528017

Designed by: Pawel Urbanski   Group: iGEM11_Warsaw   (2011-09-19)
Revision as of 22:07, 21 September 2011 by Pawel29 (Talk | contribs) (→‎Construct design)

Expression Adapter s02_20k


Construct design

To make truly standardize strength of RBS parts we propose a brand new part in synthetic biology - we call expression adapters. They are short sequences consisting of RBS, 5 bp spacer and a short (10 amino acid long) beginning of a protein. 20k is strength in Voight's virtual units.

For more info please look at Team Warsaw 2011 http://2011.igem.org/Team:Warsaw/ExpressionAdaptors/Results

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None