Coding

Part:BBa_K592001:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-21)
Revision as of 09:08, 21 September 2011 by SunLei (Talk | contribs) (→‎Source)

CcaS, green light sensor from Synechocystis sp. PCC6803


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA was extracted from Tabor's pJT122 plasmid. Two illegal internal restriction sites had to be removed in order to allow BioBrick-compatibility. The removed sites are: A1282C and A1284C to remove EcoRI site, and T1383C to remove SpeI site. Furthermore, while removing the EcoRI site, G1278T was also done to exchange a rare codon (GGG to GGT).

Source

Synechocystic sp. PCC6803, pJT122 plasmid of Jeffrey Tabor

References