Measurement

Part:BBa_K546002:Experience

Designed by: M. Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 18:17, 19 September 2011 by Youri (Talk | contribs) (User Reviews)

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Applications of BBa_K546002

This part was used as an 3-oxohexanoyl-homoserine lactone (AHL) responsive element. lux pL yields low constitutive expression which results in luxR production. Complexed with luxR, AHL binds to the luxpR promoter which drives the expression of GFP.

User Reviews

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No review score entered. Youri van Nuland

Functionality of this part was tested with a Spectramax M2 spectrophotometer. This apparatus can measure fluorescence in microplates. The response to AHL was examined by applying different concentrations of AHL: 0, 250 and 1000 nM AHL in a 200 µL of overnight culture samples susepended in PBS in a 96 wells plate. As a negative control for this experiment, part BBa_K546001 was used. This part should also respond to AHL, but without yielding a fluorescent signal.

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