Composite

Part:BBa_K584015

Designed by: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne   Group: iGEM11_KULeuven   (2011-08-16)
Revision as of 09:51, 19 September 2011 by Vinchhi (Talk | contribs)

Lambda cI and LuxR regulated hybrid promoter + Ribolock-CeaB + Terminator

We performed a PCR to get the CeaB part of the K131009 biobrick. The primer design* for this was such that it already contained the ribolock resulting in ‘Ribolock + ceaB’ as the final product. This is fused with the terminator B0015 to get ‘Ribolock + ceaB + B0015’. This was finally fused with the Lambda cI and LuxR regulated hybrid promotor to get this biobrick, ‘Lambda_cI and LuxR_regulated hybrid promotor’ + Ribolock + ceaB + B0015.

  • The primer sequence we used in the PCR reaction is -

ceaB-FW: CCGGAATTCGCGGCCGCTTCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGACGAAGAGGAGATACTAGATGAGCGGTGGCGATGGACGC

ceaB-RV: AAAACTGCAGCGGCCGCTACTAGTCTCTAGTATTACTTACCCCGATGAATATCAATAT


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None