Composite

Part:BBa_K592014:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 13:11, 18 September 2011 by Registry (Talk | contribs)

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HO1-pcyA, low expression, B1001 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the toxic nature of HO1 and pcyA enzymatic activities, we added a very weak promoter (J23113) to the PCB chromophore module.


Source

Assembled using parts from the 2011 iGEM distribution.

References