Not Released
Sample In stock
Experience: Works
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Coding
S354N_Tri

Part:BBa_K590024

Designed by: Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu   Group: iGEM11_Washington   (2011-09-15)
Revision as of 06:00, 15 September 2011 by Registry (Talk | contribs)

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Kumamolisin-As_S354N, D358G, D368H

A mutated Kumamolisin-As enzyme aimed to combat celiac disease by increased specificity for the PQLP peptide, an antigenic epitope in gliadin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 447
    Illegal NgoMIV site found at 720
    Illegal NgoMIV site found at 1248
    Illegal NgoMIV site found at 1494
    Illegal AgeI site found at 772
    Illegal AgeI site found at 1348
    Illegal AgeI site found at 1588
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None