Generator
thRS1-ChZ

Part:BBa_K537001:Design

Designed by: Natasia Kruger   Group: iGEM11_WITS-CSIR_SA   (2011-07-19)
Revision as of 18:49, 19 July 2011 by Registry (Talk | contribs)

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Theophylline Riboswitch 1-CheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The effectiveness of the theophylline riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness.



Source

The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the theophylline riboswitch.


References