Part:BBa_K678001:Design
PalcA, inducible Aspergillus nidulans promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 274
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
Source
Aspergillus nidulans genomic DNA
References
(1) Lubertozzi, D., and Keasling, J.D.: Marker and promoter effects on heterologous expression in Aspergillus nidulans. Appl Microbiol Biotechnol (2006) 72: 1014–1023.
(2) Creaser, E.H., Porter, R.L., Britt, K.A., Pateman, J.A. and, Doy, C.H.: Purification and preliminary characterization of alcohol dehydrogenase from Aspergillus nidulans. Biochem. J. (1985) 225: 449-454.
(3) Bailey, C., and Arst Jr., H.N.: Carbon catabolite repression in Aspergihs nidulans. Eur. J. Biochem. (1975) 51: 573-577.
(4) Waring, R.B., May, G.S., and Morris, N.R.: Characterization of an inducible expression system in Aspergillus nidulans using alcA and tuhulin- coding genes. Gene, (1989) 79: 119-130.
(5) Romero, B., Turner, G., Olivas, I., Laborda, F., and De Lucas, J.R.: The Aspergillus nidulans alcA promoter drives tightly regulated conditional gene expression in Aspergillus fumigatus permitting validation of essential genes in this human pathogen. Fungal Genetics and Biology (2003) 40: 103–114.