Composite

Part:BBa_M36701

Designed by: Katie Lund   Group: Stanford BIOE44 - S11   (2011-05-03)
Revision as of 19:48, 3 May 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Pyruvate Sensor with Gemini Actuator

Constitutive promoter, RBS, IclR gene, terminator, aceBAK promoter, RBS, Gemini, terminator. This is designed so that the IclR gene is synthesized on the same scale as the aceBAK promoter instead of relying on IclR naturally in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 679
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2022


[edit]
Categories
Parameters
None