DNA

Part:BBa_K416002:Design

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-06-01)
Revision as of 00:09, 28 October 2010 by RussellDurrett (Talk | contribs) (→‎Source)

36 Base Pair LoxP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a LoxP site which is the target of Cre Recombinase. It is normally 34-bp, but we added 2bp to the 3' end so that the part can be assembled within a transcribed region of our system. The 2bp added do not cause a stop codon and should not interfere with transcription.

Source

We used the 34-bp LoxP Site from the 2010 iGEM initial distribution, then rebiobricked it using a reverse primer that contained the extra 2bp.

References

Sauer, Brian. Functional Expression of the cre-lox Site-Specific Recombination System in the Yeast Saccharmoyces cerevisiae Molecular and Cellular Biology, June 1987, p. 2087-2096 0270-7306/87/062087-10$02.00/0 Copyright C 1987, American Society for Microbiology

Nagy, Andras. Cre recombinase: The universal reagent for genome tailoring Genesis 26, 2000, p. 99-109 0.1002/(SICI)1526-968X(200002)26:2<99::AID-GENE1>3.0.CO;2-B US: http://dx.doi.org/10.1002/(SICI)1526-968X(200002)26:2<99::AID-GENE1>3.0.CO;2-B Copyright C 2000 Wiley-Liss, Inc