Part:BBa_K300010
PoPS-based self-inducible device
- This is a PoPS-in/PoPS-out device.
The luxR gene (BBa_C0062) is constitutively produced by the BBa_R0040 promoter and it can activate the lux pR in presence of the autoinducer 3-oxo-C6-homoserine-lactone (3OC6HSL or simply HSL). The PoPS input regulates the production of luxI gene (BBa_C0061). It encodes for the LuxI enzyme, which is able to produce HSL. The produced HSL can diffuse in the growth media of the cells that express LuxI. The lux pR produces a PoPS output when HSL reaches a critical concentration.
This device can be specialized by assembling a promoter upstream and a promoterless expression system with the gene of interest downstream. When a cell population expresses luxI, the concentration of HSL is an increasing function of cell culture density and so the induction of the lux pR promoter occurs only when the cells reach a threshold density.
In this way, the upstream promoter autoinduces the production of the target protein at a critical culture density, depending on the HSL synthesis rate. The HSL synthesis rate can be tuned by assembling promoters of different strengths upstream of luxI.
This enables the construction of a library of self-inducible devices capable of starting the target protein production at a predictable culture density.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 658
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1813
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