Part:BBa_K427007

Ter PBadweak Mu C Ter Pmom GFP cassette

This part was used for the characterization of the Mu C sensitivity tuner. It is composed of seven biobricks starting with Ter PBadweak, which is the part created by British Columbia team in 2007 starts with a terminator and a promoter that respond to high concentrations of Arabinose. It is followed by sequence coding for C protein which inter activates the Pmom promoter and this enhance the PoPS activity. Then is followed by the GFPcassette sequence a biobrick.

This part has no special safety considerations

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 228
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 168
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1542
  

References

BBa_F2620 Parts Registry entry with characterisation.

Jiang, Y. a. (2008). Regional mutagenesis of the gene encoding the phage Mu late gene activator C identifies two separate regions important for DNA binding. NucleicAcidsResearch, 6396–6405.

Kumaraswami, M., & Howe, M. a.-W. (2004). Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.