Templates/Cambridge-fireflycolour
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Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase]. Then a {{{substitution}}} mutation was used to create a {{{colour}}} bioluminescence.
Pictures
![](/wiki/images/f/f2/300px-Cambridge-Wed.jpg)
Figure 1 - E.Coli (Invitrogen TOP 10) cells transformed with BBa K325909 (blue light bulb) and BBa 325219 (red light bulb)
1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]