Device

Part:BBa_K302035:Design

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-25)
Revision as of 01:15, 26 October 2010 by Swoodhouse (Talk | contribs) (→‎Design Notes)

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Sucrose-limitation induced kill switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 363
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Sequence selected based on the work of Pellegrini at al. and Shimotsu and Henner in the papers below.
  • Organisms with this device integrated must be grown on 10% sucrose media to be viable.
  • Should be integrated at the sacB locus to prevent native Levansucrose glue production (to use in conjunction with Levansucrose glue, use part BBa_K302030).

Source

Bacillus Subtilis 168 strain

References

Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol 56: 1139–1148.

Shimotsu, H., and Henner, D. J. (1986) J. Bacteriol. 168, 380–388