Coding

Part:BBa_K302033:Design

Designed by: Philip Hall   Group: iGEM10_Newcastle   (2010-10-25)
Revision as of 23:51, 25 October 2010 by PhilipHall (Talk | contribs) (→‎Design Notes)

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mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence selected based on the work of Pellegrini at al. in the paper below. Recommended double TAA stop codon added.

Source

Bacillus Subtilis 168 strain

References

Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol 56: 1139–1148.