Composite

Part:BBa_K322140:Design

Designed by: Richard Partridge-Hicks and Maria Kowal   Group: iGEM10_Edinburgh   (2010-10-07)
Revision as of 18:32, 7 October 2010 by WillR (Talk | contribs) (→‎Design Notes)

lac promoter + luxAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1657


Design Notes

Source

J33207 The DNA was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). The annotation shown here is based on that associated with this Genbank entry. The sequence shown here is derived by sequencing the construct. References

K322139 Source: genomic DNA of Xenorhabdus (Photorhabdus) luminescens Hb (actually, we used our BioBrick from last year, BBa_K216016 as template).

References