Device

Part:BBa_K331000:Design

Designed by: Lisza Bruder   Group: iGEM10_Lethbridge   (2010-06-28)
Revision as of 01:04, 29 June 2010 by Liszabruder (Talk | contribs) (→‎Source)

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IPTG inducible Lumazine Synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We followed Seebeck et al. and mutated Arg83, Thr 86, Thr 120, and Gln123 to glutamates


Source

Sequence from Auquifex aeolicus

References

Seebeck, F. P., Woycechowsky, K. J., Zhuang, W., Rabe, J. P., and Hilvert, D., (2006). A simple tagging system for protein encapulation. J. Am. Chem. Soc. 128, 4516-4517