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Part:BBa_K416000:Design

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-05-28)
Revision as of 18:47, 16 June 2010 by RussellDurrett (Talk | contribs) (→‎References)

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Aga2 CDS Responsible for Yeast Surface Display


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have removed the STOP codon of this part to allow for N- and C-terminal Aga2 fusions, which have both been proven to occur efficiently (Refer to Wang, z. et. al. : Protein Engineering, Design & Selection, Vol. 18 No. 7 pp. 337-343, 2005.

Source

This parts was biobricked from the pCTCON2 plasmid which was sent to us by Dr. D. K. Wittrup at MIT.

References

Gai SA, Wittrup KD. 2007. Yeast surface display for protein engineering and characterization. Curr Opin Struct Biol 17 (4) 467-473.

Hackel BJ, Kapila A, Wittrup KD. 2008. Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis and loop shuffling. J. Mol Biol. 381: 1238-1252.