Tag

Part:BBa_K416000

Designed by: Russell Durrett   Group: iGEM10_NYU   (2010-05-28)
Revision as of 18:51, 16 June 2010 by RussellDurrett (Talk | contribs)

Aga2 CDS Responsible for Yeast Surface Display

In order to extracellularly display proteins on the yeast cell surface, the protein is fused to this Aga2 protein via a flexible linker. Once secreted, the Aga2 protein binds to the Aga1 protein on the yeast surface, anchoring the fusion onto the cell surface. Because the Aga2 protein must bind Aga1 to display, the chassis yeast strain must also express Aga1.

The STOP codon at the end of the gene was removed from this CDS to allow for both N- and C-terminus fusions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain/localization
Parameters
None