Composite
Part:BBa_K5335023
Designed by: Ruichen Shen Group: iGEM24_HZAU-China (2024-09-28)
A part (including a reporter gene) that induces and regulates downstream gene expression by salicyli
This part includes the LysR-type regulator NahR, the NahR binding sequence and the inducible promoter Psal, and a cyan fluorescent protein, amCyan. In this part, Amcyan was used as a reporter gene, and the expression level could be measured by light excitation at 453nm and detection at a wavelength of 486nm. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 786
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1855 - 1000COMPATIBLE WITH RFC[1000]
Introduction
Usage and Biology
Characterization
[edit]
Categories
Parameters
None |