Coding

Part:BBa_K5097009

Designed by: Elber Lopez Hernandez, Kelly Acen, Runyararo Chaora   Group: iGEM24_Oneonta   (2024-09-27)
Revision as of 13:28, 28 September 2024 by Elberlopezh (Talk | contribs)


SpeA (arginine decarboxylase)

This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase. This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine. The 2024 iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1167
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None