Part:BBa_K5459002
Through the experiment, we concluded that our designed single-plasmid system can also generate a fluorescent signal in a copper-rich environment. This system utilizes the endogenous regulatory protein of E.coli, meaning the expression level of *E. coli*'s own regulatory protein is sufficient to activate the additional reporter system. In future designs of regulatory mechanisms, if the regulatory protein is naturally present in *E. coli*, there may be no need to encode this protein on a plasmid for exogenous expression. Instead, we can attempt to directly use the regulatory protein expressed from the genome, thereby reducing the burden on the engineered bacteria.
Upon comparison, we found that the results of the dual-plasmid system were not as strong as those of the single-plasmid system in terms of signal strength. However, the overall curve became steeper, and the induction threshold for low copper ion concentrations was lower. This leads us to speculate that different expression levels of CueR might cause different system responses.
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